Next generation sequencing basics – RNA

RNA-sequencing (RNA-seq) is a technique that utilises next-generation sequencing to investigate gene expression in cells by quantifying the presence and amount of RNA reads within a sample. This allows scientist to explore the cellular transcriptome (total cellular RNA content including mRNA, ncRNA, miRNA, etc.), evaluate changes in gene expression (e.g. caused by changes in environmental […]

Integrating scRNA-seq and scATAC-seq data

Single cell RNA sequencing (scRNA-seq) has exemplified recent advances in the anlaysis of transcriptomic heterogeneity, enabling the characterisation of gene expression in individual cells. The utility of scRNA-seq data can be further enhanced through combination with other technologies that have been developed to capture other molecular measurements. For example, single cell assay for transposase-accessible chromatin […]

Single cell RNA Sequencing (scRNASeq)

A technique used to study gene expression in single cells. In contrast to bulk RNASeq methods, single cell RNASeq can capture gene expression information in individual cells and reveal cell-to-cell variation within a population. Author: Ryan Hagan (ESR6)