Experimental design for single cell analysis

It is challenging to create generalizable designs for single-cell transcriptomic experiments because each one requires the user to make informed decisions in order to obtain interpretable results. These include the selection of sample types, cell numbers and preparation methods; the choice of scRNA-seq techniques and sequencing parameters; and the design of computational analysis strategies to […]

Integrating scRNA-seq and scATAC-seq data

Single cell RNA sequencing (scRNA-seq) has exemplified recent advances in the anlaysis of transcriptomic heterogeneity, enabling the characterisation of gene expression in individual cells. The utility of scRNA-seq data can be further enhanced through combination with other technologies that have been developed to capture other molecular measurements. For example, single cell assay for transposase-accessible chromatin […]