Identifying differentially expressed genes by scRNA-seq

Single-cell RNA sequencing (scRNA-seq) comprises a state-of-the-art technology for analysing the gene expression profile of individual cells from a complex or even a uniform specimen. Differential gene expression analysis and Uniform Manifold Approximation and Projection (UMAP) plots may reveal transcriptomic differences among cell populations, pointing out genes that are either upregulated or downregulated in relation […]


Uniform Manifold Approximation and Projection (UMAP) is a dimensionality reduction technique to construct graphs showing the complexity of specimens and how the cell clusters diverse between each other according to a selected group of features (i.e. genes). Principal component analysis (PCA) prior UMAP plotting speeds up the computational process. It is closely related with t-distributed […]