Single cell transcriptomics – a gerneric explanation of common steps

Single-cell RNA sequencing (scRNA-seq) has significantly advanced our knowledge of biological systems by studying the single cell transcriptome. Compared to bulk RNA-seq, scRNA-seq is able to study cellular heterogeneity. BDTM Rhapsody Steps of single cell transcriptomics Capture single cells Magnetic barcoded beads to microwells (one bead to one cell) Cell lysis to hybridize mRNA´s onto […]