Lentiviral vectors

A common vehicle used extensively in gene therapy is the lentiviral vector (LV). The LV is a replication-defective viral vector, generated from HEK293T cells (human embryonic kidney cells) after the transfection with plasmids (DNA molecules) dedicated for the assembling of particular viral parts (envelope, capsid, nucleoproteins, viral RNA, reverse transcriptase, integrase etc). The purified particles […]