ESR9: Theofilor Filippos Charitidis

Host: B6 Paul-Ehrlich-Institut / Supervisor: Prof. Dr. Christian Buchholz

Individual Project

Following CAR T cell generation using LV via single cell transcriptomics.


ESR 9 will investigate the molecular basis for the alteration of CAR T cell phenotypes during the manufacturing process. They will correlate the activation status, exhaustion level, phenotype and functional activity with gene and protein expression of single T cells after each manufacturing step.

Scientific Background

Obtaining my Bachelor Degree in Biology from the Aristotle University of Thessaloniki, in Greece, where I was also trained in cancer cell cultures and cytotoxicity assays in the “Theagenio” Anticancer Hospital, I conducted my dissertation project in the “George Papanikolaou” General Hospital, evaluating the oxidation stress of post-thawed umbilical cord blood stem cells and the correlation with the CD34+ derived microparticles. Furthermore, I participated in an Erasmus+ Internship Programme in the Lund University, in Sweden, assessing the binding features of a telomere-binding protein subunit. Recently, I obtained my Master Degree in Immunology and Inflammation from the University of Copenhagen, in Denmark. I conducted my thesis project in LEO Pharma A/S, where I had the opportunity to develop a mouse model of induced psoriasis-like inflammation.


  • DOI: Charitidis F. (ESR9), Adabi E. (ESR10), Thalheimer F.B., Clarke C., Buchholz C.J., et al. (2021), Monitoring CAR T cell generation with a CD8-targeted lentiviral vector by single-cell transcriptomics. Molecular Therapy, Volume 23, P359-369, December 10, 2021.
  • Two further publication is being prepared for submission to a peer-reviewed journal in 2023.


  • Oral presentation: PEI PhD Conf. 2020, Lange, Germany, 29.01.2020.
    BD Rhapsody single-cell RNA-seq technical workflow and application on CAR T cell therapy.
  • Oral presentation: 25th American Society of Gene & Cell Therapy (ASGCT), Washington, USA, 03.02.22.
    Enhanced CAR T Cell Generation by CD8-LV Through Alleviating Antiviral Mechanisms with mTOR Inhibitors.
  • Poster presentation: PEI Retreat, Langen, Germany, 17.01.20.
    Characterization of CAR T cells by single-cell expression profiling.
  • Poster presentation: 2nd Rhein-Main Cancer Retreat 2020, Virtual.
    Characterization of CAR T cells by single-cell expression profiling.
  • Poster presentation: 24th American Society of Gene & Cell Therapy (ASGCT), Virtual, 14.09.20.
    Analyzing the Effectiveness of CD8-Receptor Targeted Transduction for CAR T Cell Therapy Using Single Cell Transcriptomics.
  • Poster presentation: 3rd International Conference on Lymphocyte Engineering (ICLE), München, Germany, 29.01.20.
    Monitoring early transcriptional profiles of CAR T cell products generated with conventional or CD8-targeted lentiviral vectors.