ESR10: Elham Adabi

Host: B6 Paul-Ehrlich-Institut / Supervisor: Prof. Dr. Christian Buchholz

Individual Project

Following CART T cell generation via single cell transcriptomics and barcoded antibody technology.


ESR10 will apply the use of antibodies in single cell RNA technology (AbSeq) to identify the subtypes and phenotypes of T cells that are preferentially transduced by lentiviral vectors specifically targeted to subtypes of human T cells.

Scientific Background

I obtained my Master Degree in medical biotechnology from Tehran University of Medical Sciences, Iran. During my masters’ thesis, we investigated a strategy to genetically engineer IL-2, in order to improve the efficacy and pharmacokinetic properties of this cytokine, as a passive cancer immunotherapy agent. Afterwards, as a research assistant in Endocrinology and Metabolism research institute in Tehran, I performed gene expression analysis to set novel biomarkers, and I also gained some experience in the maintenance of Zebrafish Lab. Then, I joined Hannover biomedical research school, where I focused on defining the role of gamma delta T-cells in EBV associated malignancies.


  • DOI: Charitidis F. (ESR9), Adabi E. (ESR10), Thalheimer F.B., Clarke C., Buchholz C.J., et al. (2021), Monitoring CAR T cell generation with a CD8-targeted lentiviral vector by single-cell transcriptomics. Molecular Therapy, Volume 23, P359-369, December 10, 2021.
  • DOI: Ho N., Jamali A., Braun A., Adabi E. (ESR10), Thalheimer F.B., Buchholz C.J. (2022), Early induction of cytokine release syndrome by rapidly generated CAR T cells in a preclinical mouse model.
  • Two further publication is being prepared for submission to a peer-reviewed journal in 2023.


  • Oral presentation: TUDA PhD symposium, Virtual, 02.10.20.
    Following CAR T cell generation via single cell transcriptomics and barcoded antibody technology.
  • Oral presentation: PEI conference 2022, Langen, Germany, 02.10.22.
    Tracking CAR gene delivery with conventional and T cell targeted lentiviral vectors by single cell multiomics.
  • Poster presentation: European Society for Gene and Cell Therapy 29th Congress, Edinburgh, Belgium, 11.10.22.
    Tracking CAR gene delivery with conventional and T cell-targeted lentiviral vectors by single-cell multiomics.