ESR6: Ryan Hagan

Individual Project

Understanding CHO cell heterogeneity to enable increased production of recombinant therapeutic proteins.

STACCATO Research

Ryan will focus  on  utilising single  cell  analysis  to  understand  the  relationship between  heterogeneity  and  bioprocess  performance  for  recombinant  protein  production  in  CHO  cells.

Scientific Background

Ryan holds a 1st class master’s degree in Biochemistry from Queen’s University Belfast (QUB), Northern Ireland – graduating in 2017. During his degree, Ryan successfully completed an internship at stratified medicine company Almac Diagnostics (Almac Group LTD). Ryan completed his master’s project under the supervision of Professor Manuel Salto-Tellez at the Centre for Cancer Research and Cell Biology at QUB, focusing on the analysis of prognostic immune biomarkers in colorectal cancer and validation of a novel digital image analysis tool. Additionally, Ryan gained experience in the public health sector working as an epidemiological scientist, prior to joining the STACCATO project.

Publications

• DOI: Tzani I., Monger C., Motheramgari K., Hagan R. (ESR6), et al. (2020), Subphysiological temperature induces pervasive alternative splicing in Chinese hamster ovary cells. Biotech. & Bioeng. Vol. 117, 8. Iss. Aug:20, 2486-2503.
• DOI: Tzani I., Herrmann N., Carillo S., Hagan R. (ESR6), et al. (2021), Tracing production instability in a clonally derived CHO cell line using single-cell transcriptomics. Biotech. & Bioeng. Vol. 118, 5. Iss. May:21, 2016-2030.
• DOI: Virgolini N. (ESR8), Hagan R. (ESR6), Correia R., Silvano M. (ESR7), et al. (2022), Transcriptome analysis of Sf9 insect cells during production of recombinant AAV. Biotech. Journ. 2200466. November 19, 2022.
• DOI: Virgolini N. (ESR8), Silvano M. (ESR7), Hagan R. (ESR6), et al. (2022), Impact of dual baculovirus infection on the Sf9 insect cell transcriptome during AAV production using single-cell RNA-seq. Authorea. December 16, 2022.
• Two further publications are being prepared for submission to peer-reviewed journals in 2023.

Conferences

• Oral presentations: ESACT Frontiers, Vienna, Austria, 28.10.22.
  Interrogating production instability in CHO cells using single cell ATAC and RNA sequencing.
• Oral presentations: European Society for Animal Cell Culture Technology (ESACT), Lisbon, Portugal, 26. – 29.06.22.
  Single CHO cell ATAC-seq.
• Poster presentation: European Society for Animal Cell Culture Technology (ESACT), Lisbon, Portugal, 26. – 29.06.22.
  Integrated analysis of chromatin accessibility and gene expression in Chinese hamster ovary cells.
• Poster presentation: UCD SCBE Industry-Research event, Dublin, Ireland, 09.09.22.
  Integrated analysis of chromatin accessibility and gene expression in Chinese hamster ovary cells.
• Ryan presented results from his masters project at the Belfast Pathology conference (2017), winning the best poster in the ‘technical advances’ category. This was subsequently included for work published in peer reviewed journal Histopathology (doi: 10.1111/his.13516).