ESR10: Elham Adabi

Individual Project

Following CART T cell generation via single cell transcriptomics and barcoded antibody technology.

STACCATO Research

ESR10 will apply the use of antibodies in single cell RNA technology (AbSeq) to identify the subtypes and phenotypes of T cells that are preferentially transduced by lentiviral vectors specifically targeted to subtypes of human T cells.

Scientific Background

I obtained my Master Degree in medical biotechnology from Tehran University of Medical Sciences, Iran. During my masters’ thesis, we investigated a strategy to genetically engineer IL-2, in order to improve the efficacy and pharmacokinetic properties of this cytokine, as a passive cancer immunotherapy agent. Afterwards, as a research assistant in Endocrinology and Metabolism research institute in Tehran, I performed gene expression analysis to set novel biomarkers, and I also gained some experience in the maintenance of Zebrafish Lab. Then, I joined Hannover biomedical research school, where I focused on defining the role of gamma delta T-cells in EBV associated malignancies.

Publications

• DOI: Charitidis F. (ESR9), Adabi E. (ESR10), Thalheimer F.B., Clarke C., Buchholz C.J., et al. (2021), Monitoring CAR T cell generation with a CD8-targeted lentiviral vector by single-cell transcriptomics. Molecular Therapy, Volume 23, P359-369, December 10, 2021.
• DOI: Ho N., Jamali A., Braun A., Adabi E. (ESR10), Thalheimer F.B., Buchholz C.J. (2022), Early induction of cytokine release syndrome by rapidly generated CAR T cells in a preclinical mouse model.
• Two further publication is being prepared for submission to a peer-reviewed journal in 2023.

Conferences

• Oral presentation: TUDA PhD symposium, Virtual, 02.10.20.
  Following CAR T cell generation via single cell transcriptomics and barcoded antibody technology.
• Oral presentation: PEI conference 2022, Langen, Germany, 02.10.22.
  Tracking CAR gene delivery with conventional and T cell targeted lentiviral vectors by single cell multiomics.
• Poster presentation: European Society for Gene and Cell Therapy 29th Congress, Edinburgh, Belgium, 11.10.22.
  Tracking CAR gene delivery with conventional and T cell-targeted lentiviral vectors by single-cell multiomics.