STACCATO Wiki online

Our Wiki is online! You can now find explanations for terms related to STACCATO on our website. The content is produced by our ESRs and PIs and will continue to grow. We have also introduced a search function to the website so you can easily search the Wiki and our entire website for the information […]

Single cell proteomics

Like the field of Proteomics, Single-Cell Proteomics encompasses all aspects of protein study in single-cells, including but not limited to protein structural analysis, identification, abundance and the qualitative and quantitative characterization of associated Post Translational Modification (PTMs). Author: Marco Romero (ESR3)

CAR T cells

CAR T cells are genetically modified T cells, with the ability to express special receptors called chimeric antigen receptors (CARs). CARs are designed for a specific surface protein on cancer cells, and they enable T cells to efficiently recognize specific cancer cells. Author: Elham Adabi (ESR10)

Human embryonic stem cells

Human embryonic stem cells are derived from the inner cell mass of the blastocyst stage of a human embryo. These cells are pluripotent, which means they are able to differentiate into every cell type in the body and therefore have the possibility to differentiate into all three germ layers Mesoderm, Ecoderm and Endoderm. Human embryonic […]


Mitochondria contain multiple copies of mitochondrial DNA (mtDNA). When all copies are identical, the mitochondria is said to exist in “homoplasmy”. Sometimes, a copy of mtDNA has a variant (e.g. a C instead of a T). When variant mtDNA co-exists with wildtype mtDNA, the mitochondrion exists in a state known as “heteroplasmy”. If the level […]

Gene therapy

Gene therapy is a new class of treatments that promise to treat and possibly cure many debilitating diseases. In contrast to conventional treatments, gene therapy treats the cause of the disorder rather than just the symptoms. Gene therapy functions by inserting new genetic material, either DNA or RNA, into a patient’s damaged cells. The most […]

Virus-like particle

Virus-like particles (VLPs) are multiprotein structures that mimic the organization and conformation of authentic native viruses but lack the viral genome, thus making them safe and effective vaccine candidates. VLPs can present conformational epitopes similar to the native virus in a highly repetitive fashion; hence, humoral and cellular immune responses are expected to be significantly […]

Single cell RNA Sequencing (scRNASeq)

A technique used to study gene expression in single cells. In contrast to bulk RNASeq methods, single cell RNASeq can capture gene expression information in individual cells and reveal cell-to-cell variation within a population. Author: Ryan Hagan (ESR6)

Baculovirus Expression Vector System (BEVS)

BEVS is a fast and extensively used expression system in which a gene of interest is overexpressed in insect cells upon infection with a genetically modified baculovirus, in order to produce a variety of recombinant proteins, including virus-like particles and viral vectors. Author: Nikolaus Virgolini (ESR8)

Flow cytometry

Flow cytometry is a technology that simultaneously measures and then analyses multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. These characteristics can be relative size, relative granularity/complexity, and relative fluorescence intensity. Author: Teresa Swift (ESR1)