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Dr. Jonathan Bones

B1: The National Institute for Bioprocessing Research and Training, NIBRT

Position & Role in STACCATO

Principal Investigator at the NIBRT Characterisation and Comparability Laboratory in Dublin, Ireland. Main supervisor of ESR3, Co-supervisor of ESR5.

Background

Jonathan is a Principal Investigator at NIBRT in Dublin, Ireland. Jonathan received his PhD in analytical chemistry in 2007 from Dublin City University. He undertook postdoctoral research with Prof. Pauline Rudd at NIBRT and Prof. Barry Karger at Northeastern University in Boston. Jonathan now heads the Characterisation and Comparability lab at NIBRT which is focused on the development of advanced methods for biopharmaceutical and bioprocess characterisation.

Key Publications

Füssl F, Trappe A, Cook K, Scheffler K, Fitzgerald O, Bones J. Comprehensive characterisation of the heterogeneity of adalimumab via charge variant analysis hyphenated on-line to native high resolution Orbitrap mass spectrometry. MAbs. 2018 Oct 8:1-13. [DOI: 10.1080/19420862.2018.1531664].

Smith J, Davey G, Polom K, Roviello F, Bones J. Mining the acidic serum proteome utilizing off-gel isoelectric focusing and label free quantitative liquid chromatography mass spectrometry. J Chromatogr A. 2018 Sep 7;1566:32-43. [DOI: 10.1016/j.chroma.2018.06.044].

Dorival-García N, Carillo S, Ta C, Roberts D, Comstock K, Lofthouse S, Ciceri E, D’Silva K, Kierans G, Kaisermayer C, Lindeberg A, Bones J. Large-Scale Assessment of Extractables and Leachables in Single-Use Bags for Biomanufacturing. Anal Chem. 2018 Aug 7;90(15):9006-9015. [DOI: 10.1021/acs.analchem.8b01208].

Albrecht S, Kaisermayer C, Reinhart D, Ambrose M, Kunert R, Lindeberg A, Bones J. Multiple reaction monitoring targeted LC-MS analysis of potential cell death marker proteins for increased bioprocess control. Anal Bioanal Chem. 2018 May;410(13):3197-3207. [DOI: 10.1007/s00216-018-1029-3].

Füssl F, Cook K, Scheffler K, Farrell A, Mittermayr S, Bones J. Charge Variant Analysis of Monoclonal Antibodies Using Direct Coupled pH Gradient Cation Exchange Chromatography to High-Resolution Native Mass Spectrometry. Anal Chem. 2018 Apr 3;90(7):4669-4676.[DOI: 10.1021/acs.analchem.7b05241].

Albrecht S, Kaisermayer C, Gallagher C, Farrell A, Lindeberg A, Bones J. Proteomics in biomanufacturing control: Protein dynamics of CHO-K1 cells and conditioned media during apoptosis and necrosis. Biotechnol Bioeng. 2018 Jun;115(6):1509-1520. [DOI: 10.1002/bit.26563].

Floris P, McGillicuddy N, Albrecht S, Morrissey B, Kaisermayer C, Lindeberg A, Bones J. Untargeted LC-MS/MS Profiling of Cell Culture Media Formulations for Evaluation of High Temperature Short Time Treatment Effects. Anal Chem. 2017 Sep 19;89(18):9953-9960. [DOI: 10.1021/acs.analchem.7b02290].